Keratitis is a condition in which the cornea of the eye becomes inflamed. It is caused by Bacteria, Viruses, Fungi and Parasites. This study investigate that the evaluation the clinical isolates and microbial diagnosis of keratitis causing microorganism using 16SrDNA and 18SrDNA gene sequencing for bacteria and fungi from infectious eye sample of keratitis patients. All keratitis subjects were examined with a slit lamp biomicroscope in the Eye Clinic of the General Hospital of Tiruchirappalli. The Bacteria and fungus were isolated in eye swabs of keratitis patients. The bacterial and fungal genes were sequenced by using 16S rRNA and 18S rRNA- PCR. These sequences were identified using BLASTn (Basic Local Alignment Search Tool nucleotide) and deposited in the GenBank (NCBI) with the accession numbers Staphylococcus aureus (JN378392), Staphylococcus epidermidis (HQ404365), Micrococcus sp.(HM20450), Streptococcus viridians(JN378393), Moraxella sp.(JQ039348), Propionibacterium acnes (JQ039349), Acinetobacter baumanii (JN652129), Citrobacter koseri (JN652127), Pseudomonas aeruginosa(JQ039350), Klebsiella sp.(JN652128) and flavobacterium (JN835368) whereas fungal genes such as Fusarium sp.(JQ412816), Candida albicans (JX912562), Aspergillus sp.(JX204747), Sebipora aquosa (submitted) Byssochlamys nivea (Submitted). Eleven bacterial strains and five fungal strains were identified and the results in our study may be useful for further research about molecular characterizing studies.
Loading....